莊敏    助理教授、研究員
所在學院生命科學與技術學院
研究方向蛋白質泛素修飾
聯系方式zhuangmin@@shanghaitech.edu.cn


個人簡介
2003年本科畢業于南京大學基礎學科教學強化部;2009年在美國田納西大學健康醫學中心獲博士學位;2009年至2014年在加州大學舊金山分校進行博士后研究;2014年加入上??萍即髮W生命科學與技術學院任助理教授、研究員。

主要研究內容
本課題組綜合利用生物化學、細胞生物學、蛋白質組學和結構生物學等一系列的研究手段,開發應用研究蛋白質相互作用的新方法,  并將其應用到蛋白質泛素修飾領域, 研究泛素修飾在調節細胞器功能方面的作用。

代表性論文


1.Liu Q.*, Zheng J.*, Sun W., Huo Y., Zhang L., Hao P., Wang H.#, Zhuang  M#. A Proximity-tagging System to Identify Membrane Protein-Protein  Interactions. Nature Methods 15, 715-722, 2018. (Research Highlight:  Deane, C. PUP up the volume. Nat Chem Biol 14, 903, 2018)


2.Srinivasan S.R., Cesa L.C., Li X., Julien O., Zhuang M., Shao H.,  Chung J., Maillard I., Wells J.A., Duckett C., Gestwicki J.E. Heat shock protein  70 (Hsp70) suppresses RIP-1 dependent apoptotic and necroptotic cascades. Mol  Cancer Res 16(1):58–68, 2017.


3.Hill Z.B., Pollock S.B., Zhuang M., Wells J.A. Direct proximity  tagging of small molecule protein targets using an engineered NEDD8 ligase. J.  Am. Chem. Soc. 138(40): 13123-13126, 2016.


4. Julien O.*, Zhuang M.*, Witta A.P., O’Donoghue A.J.,  Knudsen G.M., Craik C.S., Wells J.A.. Quantitative MS-based enzyme of caspases  reveals distinct protein substrate specificities, hierarchies, and cellular  roles. PNAS 113(14) E2001-E2010, 2016. (*equal  contribution)

5. Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo  Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X.,  Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E.,  Stricker T., Tian Y., Schulman B.A., Liu J., White K.P.. SPOP promotes  tumorigenesis by acting as a key regulatory hub in kidney cancer. Cancer  Cell 25, 1-14, 2014.

6. Zhuang M., Guan S., Wang H.,  Burlingame A.L., and Wells J.A.. Substrates of IAP ubiquitin ligases identified  with a designed orthogonal E3 ligase, the NEDDylator. Mol Cell 49,  273-282, 2013. (Faculty of 1000: http://f1000.com/prime/717975954)

7.  Crawford E.D., Seaman J.E., Agard N., Hsu G.W., Julien O., Mahrus S., Nguyen H.,  Shimbo K., Yoshihara H.A., Zhuang M., Chalkley R.J., Wells J.A.. The  DegraBase: a database of proteolysis in healthy and apoptotic human cells.  Mol Cell Proteomics 12, 813-824, 2013.

8. Zhuang M.,  Calabrese M.F., Liu J., Waddell M.B., Nourse A., Hammel M., Miller D.J., Walden  H., Duda D.M., Seyedin S.N., Hoggard T., Harper J.W., White K.P., Schulman B.A..  Structures of SPOP-substrate complexes: insights into molecular architectures of  BTB-Cul3 ubiquitin ligases. Mol Cell 36, 39-50, 2009. (Faculty of 1000:  http://f1000.com/prime/1166991)

9. Jin Y., Zhuang M. &  Hendershot L.M.. ERdj3, a luminal ER DnaJ homologue, binds directly to unfolded  proteins in the mammalian ER: identification of critical residues.  Biochemistry 48, 41-49, 2009.

10. Huang D.T., Zhuang M.,  Ayrault O., Schulman B.A.. Identification of conjugation specificity  determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2.  Nat Struct Mol Biol 15, 280-287, 2008.

11. Huang D.T., Hunt H.W.,  Zhuang M., Ohi M.D., Holton J.M., Schulman B.A.. Basis for a  ubiquitin-like protein thioester switch toggling E1-E2 affinity. Nature  445, 394-398, 2007. (Faculty of 1000:  http://f1000.com/prime/1060912)

12. Huang D.T., Paydar A., Zhuang  M., Waddell M.B., Holton J.M., Schulman B.A.. Structural basis for  recruitment of Ubc12 by an E2 binding domain in NEDD8's E1. Mol Cell 17,  341-350, 2005. 


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